Journal: International Journal of Molecular Sciences

Article Title: Choline-Sigma-1R as an Additional Mechanism for Potentiation of Orexin by Cocaine

doi: 10.3390/ijms22105160

Figure Lengend Snippet: OXA (17-33) A increases [Ca 2+ ] i via choline-Sigma-1R-dependent mechanism. ( A ) Illustration of average Ca 2+ transients (± SD) induced by OXA (17-33) (10 nM) alone (left) and in the presence of VU0155056 (1 μM), PLD inhibitor (middle), and BD1047 (50 µM) or NE-100 (5 µM), Sigma-1R antagonists (right). ( B ) Comparison of the amplitude of the increase in [Ca 2+ ] i (average + SD) in each condition. Inhibition of PLD or antagonism of Sig-1R reduces the Ca 2+ response elicited by OXA (17-33) (10 nM); p < 0.05 as compared to amplitude of [Ca 2+ ] i increase produced by OXA (17-33) (*) or produced in the presence of the inhibitors ( # ) ( n = 6 neurons/condition).

Article Snippet: VU0155056, a PLD inhibitor [ ], was purchased from Avanti Polar Lipids (Alabaster, AL, USA).

Techniques: Comparison, Inhibition, Produced

Journal: Microbiology Spectrum

Article Title: Evaluating the health risk of probiotic supplements from the perspective of antimicrobial resistance

doi: 10.1128/spectrum.00019-24

Figure Lengend Snippet: Antibiotics used in this study

Article Snippet: Piperacillin sodium , −20°C , 59703-84-3 , H2119223 , Aladdin Scientific (Shanghai, China).

Techniques:

Journal: bioRxiv

Article Title: SK2/3 CHANNELS COUPLE WITH T-TYPE CA 2+ CHANNELS TO GATE SPINAL LOCOMOTOR RHYTHM GENERATION

doi: 10.64898/2026.03.19.712770

Figure Lengend Snippet: a-b Representative ventral root recordings showing the effect of the broad-spectrum SK activator 1-EBIO ( a ; 5 mM) and the SK2/3-selective activator CyPPA ( b ; 0.3-0.4 mM) on ongoing fictive locomotion induced by NMDA/5-HT (5/10 µM). Top left in a and b : Schematic of the isolated spinal cord preparation illustrating focal drug puff application onto rhythmogenic segments (L 1 -L 2 ) and bilateral L5 ventral root recordings (L5R, L5L). Bottom: Raw ventral root activity (black) superimposed with low-pass filtered envelopes (blue for 1-EBIO, orange for CyPPA); vertical arrows indicate puff onset. Top right: Expanded time windows ( a’ , b’ ) of the recordings shown below, highlighting rhythm suppression. c-d Representative recordings showing the induction of locomotor-like activity in quiescent preparations (subliminal NMDA/5-HT: 0-1/10 µM) by the SK2/3 inhibitor tamapin ( c ; 1 µM) or the T-type calcium channel blocker nickel ( d ; 10-20 µM). The experimental layout and display are identical to a, b . Low-pass filtered envelopes are shown in purple (tamapin) and teal (nickel). Arrows indicate puff onset.

Article Snippet: Alomone: tamapin (5-10 nM), 1-EBIO (200 μM), CyPPA (1-10 μM), and Tram-34 (5 μM).

Techniques: Isolation, Activity Assay

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: The Chemopreventive Effect of Tanacetum Polycephalum Against LA7-Induced Breast Cancer in Rats and the Apoptotic Effect of a Cytotoxic Sesquiterpene Lactone in MCF7 Cells: A Bioassay-Guided Approach.

doi: 10.1159/000430273

Figure Lengend Snippet: Fig. 4. Immunohistoche- mistry staining of Bax, Bcl-2, P53 and Caspase 3 proteins. A) Tumor control B) Low dose TPHE treatment C) High dose TPHE treatment D) tamoxifen treatment. The analysis of Bax and Bcl-2 shows an up-regulation of Bax, p53 and caspase 3 and down-regulation of Bcl-2 which revealed the induc- tion of apoptosis through mitochondrial pathway. Ma- gnification 40x.

Article Snippet: The membrane was incubated overnight with specific primary antibodies, namely, Bax: 1:10,000 (Cat: sc-493); Bcl-2: 1:10,000 (Cat: sc492); Caspase 7: 1:10,000 (Cat: sc-33773); Caspase 9: 1:10,000 (Cat: sc-7885); p21: 1:10,000 (Cat: sc-397); p27: 1:10,000 (Cat: sc-528) and β-actin, 1:10,000 (Cat: sc-7210) (Santa Cruz Biotechnology, Inc., CA, USA).

Techniques: Staining, Control

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: The Chemopreventive Effect of Tanacetum Polycephalum Against LA7-Induced Breast Cancer in Rats and the Apoptotic Effect of a Cytotoxic Sesquiterpene Lactone in MCF7 Cells: A Bioassay-Guided Approach.

doi: 10.1159/000430273

Figure Lengend Snippet: Fig. 11. Q-PCR analysis of apoptosis- and cell cycle-associated molecules, viz. Bcl-2, Bax, caspase 9, caspase 7, p21 and p27. MCF7 cells were exposed to HDZC at IC50 concentration for 12, 24 and 48 h. The mRNA ex- pression of genes was represented after normalization with β-actin housekeeping gene. The data represent the means ± SEM of three independent experiments. *p<0.05 compared with the control group.

Article Snippet: The membrane was incubated overnight with specific primary antibodies, namely, Bax: 1:10,000 (Cat: sc-493); Bcl-2: 1:10,000 (Cat: sc492); Caspase 7: 1:10,000 (Cat: sc-33773); Caspase 9: 1:10,000 (Cat: sc-7885); p21: 1:10,000 (Cat: sc-397); p27: 1:10,000 (Cat: sc-528) and β-actin, 1:10,000 (Cat: sc-7210) (Santa Cruz Biotechnology, Inc., CA, USA).

Techniques: Concentration Assay, Control